Hygromycin-Resistant Bacterial Selection: Selects transformants carrying hygromycin-resistance plasmids in Escherichia coli, Bacillus subtilis, and Agrobacterium tumefaciens.

Cloning and Transformation Screening: Recovers colonies after heat-shock or electroporation and enables insert verification via colony PCR.

Plasmid Maintenance and Propagation: Preserves selective pressure to maintain hygromycin-marked plasmids during routine passaging.

CRISPR-Edited Clone Selection: Enriches bacterial clones harboring hygromycin-selectable edits or integration cassettes.

Library Construction and Screening: Supports selection and amplification of hygromycin-marked DNA or gRNA libraries.

Colony Isolation and CFU Enumeration: Enables streaking for single colonies and counting CFUs under hygromycin selection.

Expression Strain Pre-Selection: Establishes clonal starter colonies for protein expression vectors with hygromycin resistance in Escherichia coli.

Conjugation/Transconjugant Selection: Selects hygromycin-resistant recipients after bacterial mating in Escherichia coli or Agrobacterium tumefaciens.

Shuttle Vector Maintenance: Maintains hygromycin-marked shuttle plasmids propagated in Escherichia coli and transferred to Agrobacterium tumefaciens.