Antibiotic Selection of Transformants: Isolates colonies carrying gentamicin-resistance plasmids (30 µg/mL) in Escherichia coli and other Gram-negative bacteria.

Plasmid Cloning and Maintenance: Maintains vectors with gentamicin-resistance cassettes (e.g., aacC1/aac(3)) in Escherichia coli.

Colony Isolation and Streaking: Obtains single colonies under gentamicin selection for downstream workflows.

CFU Enumeration Under Selection: Quantifies viable gentamicin-resistant cells after transformation or conjugation.

Transposon Mutant Selection: Recovers mutants carrying gentamicin-marked transposons in organisms such as Escherichia coli and Pseudomonas aeruginosa.

Bacterial Conjugation/Mating Selection: Selects recipients or exconjugants acquiring gentamicin-marked plasmids.

Library Maintenance: Preserves genomic or plasmid libraries that use gentamicin resistance as the selectable marker.

Plasmid Stability Screening: Assesses retention of gentamicin-marked plasmids during serial passaging.

Phenotypic Verification of Resistance: Confirms expected gentamicin-resistance phenotypes via spot or streak assays.

Work with Diverse GentR Strains: Supports selection in organisms such as Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Salmonella enterica, Vibrio cholerae, and Agrobacterium tumefaciens.