Antibiotic Selection of Transformants: Isolates colonies of kanamycin-resistant transformants, commonly in Escherichia coli, on LB agar containing 40 µg/mL kanamycin.

Plasmid Maintenance and Propagation: Maintains selective pressure for KanR plasmids during routine streaking and passaging of hosts like E. coli and Bacillus subtilis.

Colony Isolation and Purification: Enables single-colony isolation and restreaking to obtain clonal KanR strains.

Colony PCR Screening: Supports rapid screening of KanR colonies to verify inserts, assemblies, or edits.

CFU Enumeration: Quantifies viable KanR cells after transformation, conjugation, or selection by plating serial dilutions.

CRISPR/Recombineering Selection: Selects for edits or integrations that confer kanamycin resistance via a KanR cassette.

Conjugation and Transposon Mutagenesis: Selects transconjugants or transposon insertions carrying KanR markers in hosts such as E. coli and Agrobacterium tumefaciens.

Protein Expression Workflows: Maintains KanR expression plasmids in production strains prior to induction and culture scale-up.

Strain Authentication and Stability Checks: Confirms retention of KanR vectors or genomic inserts through streaking and passage under selection.

Prepping Starter Colonies for Stocks: Generates single colonies under selection for inoculating liquid cultures and preparing glycerol stocks.