Selection of apramycin-resistant transformants: Isolates colonies harboring aac(3)IV-marked plasmids in E. coli using LB (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl, 1.5% Agar) with 100 µg/mL apramycin.

Plasmid maintenance and propagation: Stabilizes aac(3)IV-bearing plasmids and cosmids in E. coli for routine passaging on 100 mm sterile plates.

Cloning and colony screening: Supports post-ligation/Gibson assembly recovery and downstream colony PCR or sequencing from apramycin-selected E. coli colonies.

Conjugation workflows: Selects exconjugants after intergeneric mating from E. coli donors into Streptomyces using apramycin resistance.

CRISPR/Cas genome editing pipelines: Enables selection of editing vectors with aac(3)IV markers in E. coli and Streptomyces.

Bacterial strain archiving and QC: Verifies retention of apramycin-selectable markers in glycerol stocks or streak-for-isolation on LB-apramycin plates.

Library construction and screening: Maintains BAC/cosmid/fosmid or mutagenesis libraries carrying aac(3)IV in E. coli.

Agrobacterium vector maintenance: Selects plasmids with apramycin markers in Agrobacterium tumefaciens prior to plant transformation workflows.

Phage and recombineering setups: Maintains host strains with apramycin-resistant helper plasmids during lambda-Red or phage-based engineering in E. coli.

Antimicrobial susceptibility controls: Serves as a positive selection control medium when validating apramycin resistance cassettes in laboratory strains.