Bacterial Transformation and Selection: Selects transformants carrying bla-marked plasmids on LB (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl, 1.5% Agar) with 200 µg/mL carbenicillin.

Plasmid Maintenance and Propagation: Routine streaking and passaging of Escherichia coli strains harboring beta-lactamase (bla) plasmids.

Colony Screening and Clone Isolation: Picking single colonies for miniprep, restriction analysis, PCR, or sequencing of bla-bearing constructs.

Starter Culture Preparation: Inoculating isolated antibiotic-selected colonies for overnight cultures used in cloning or protein expression.

Transformation Efficiency and CFU Enumeration: Quantifying colony-forming units under 200 µg/mL carbenicillin selection.

CRISPR and Genome Editing Workflows: Selecting edited clones that retain carbenicillin-resistant editing or helper plasmids.

Conjugation/Mobilization Experiments: Selecting recipients that acquired bla-marked plasmids after mating.

Library Maintenance: Preserving pooled plasmid libraries in E. coli under beta-lactam selection to minimize plasmid loss.

Strain Archiving and Recovery: Streaking glycerol stocks of E. coli carrying bla vectors to verify viability and plasmid retention.

Antibiotic Susceptibility Controls: Verifying beta-lactamase-mediated resistance versus sensitive parental strains on a defined LB-carb background.