Antibiotic Selection: Selects chloramphenicol-resistant colonies of Escherichia coli and other bacteria at 5 µg/mL.

Plasmid Maintenance: Maintains and propagates cat-marked plasmids in E. coli under chloramphenicol selection.

Cloning and Transformation Screening: Screens transformants on selective LB agar following ligation or Gibson assemblies.

Recombinant Protein Expression Strain Maintenance: Preserves selection of expression strains carrying chloramphenicol-resistant vectors.

BAC/PAC/Cosmid Work: Selects and maintains low-copy constructs that use chloramphenicol resistance.

CRISPR/Allelic Exchange Workflows: Selects for cat cassette insertions or replacements during genome editing in E. coli.

Transposon Mutagenesis: Recovers and selects chloramphenicol-marked transposon insertion mutants.

Colony Isolation and CFU Enumeration: Isolates single colonies and quantifies viable counts under chloramphenicol selection.

Conjugation/Mating Selections: Selects transconjugants carrying chloramphenicol resistance markers.

Strain Storage and Routine Streaking: Short-term maintenance of chloramphenicol-resistant laboratory strains (e.g., E. coli).