Zeocin-based selection of Escherichia coli transformants: Isolates colonies carrying Sh ble–marked plasmids at 25 µg/mL.

Cloning and subcloning workflows: Maintains selective pressure during vector construction with Zeocin-selectable backbones.

Colony isolation and purification: Streaks for single colonies to derive clonal Zeocin-resistant lines.

Colony PCR screening: Screens Zeocin-resistant colonies to verify inserts or edits before liquid culture.

Plasmid maintenance and stability testing: Preserves recombinant plasmids by continuous selection on LB Lennox agar.

Library and variant screening: Selects desired members of mutagenesis or genomic libraries using Zeocin resistance.

Transformation efficiency benchmarking: Compares competent cell performance using Zeocin-selectable control plasmids.

Pre-culture colony picking for expression: Provides verified Zeocin-resistant clones for downstream liquid growth and protein work.

Selection of engineered Agrobacterium tumefaciens strains: Maintains Zeocin-marked binary vectors on LB agar when appropriate.

Short-term strain archiving on plates: Stores Zeocin-resistant clones for routine retrieval and passaging.