Selection of Tet-Resistant Transformants: Isolates colonies of E. coli transformed with plasmids bearing tetracycline resistance at 25 µg/mL.

Plasmid Maintenance and Propagation: Maintains tet-marked plasmids in E. coli by preventing plasmid loss during routine passaging.

Conjugation/Transduction Workflows: Selects donor-derived or phage-transduced recipients carrying tet markers following bacterial mating or phage transfer.

CRISPR/Cas Editing Selection: Enriches edited E. coli clones using tet-linked cassettes for genome engineering workflows.

Colony Isolation and PCR: Enables single-colony isolation under tetracycline selection for downstream colony PCR and sequencing.

Strain Banking Under Selection: Preserves engineered strains on plates to maintain tet-dependent constructs and reduce plasmid segregation.

CFU Enumeration of Resistant Populations: Quantifies viable counts of tetracycline-resistant cells after selection steps or stress assays.

BAC/Cosmid Library Maintenance: Supports growth of strains carrying large tet-marked constructs that require robust LB nutrition and agar support.

Agrobacterium Vector Selection: Selects tet-bearing plasmids in Agrobacterium tumefaciens on LB-based agar when appropriate for the strain.

Gram-Positive Cloning (Strain-Dependent): Facilitates selection of tet-marked constructs in compatible species such as Bacillus subtilis using LB agar.