Bacterial Transformation and Selection: Selects tetracycline-resistant transformants of E. coli at 10 µg/mL following chemical transformation or electroporation.

Plasmid Maintenance and Amplification: Maintains plasmids bearing tetR markers in E. coli cultures to ensure stable antibiotic selection.

Clone Screening and Colony Isolation: Isolates single colonies for downstream verification (e.g., colony PCR, restriction analysis) under tetracycline selection.

Conjugation/Mating Selection: Selects transconjugants carrying tetracycline-marked plasmids or transposons after bacterial mating assays.

CRISPR/Cas and Recombineering Workflows: Applies selection for edited bacterial clones when tetracycline-resistant cassettes are used as markers.

Strain Preservation and Stability Testing: Preserves and passages recombinant E. coli strains to verify retention of tet-marked constructs over generations.

Quality Control of Antibiotic Resistance Constructs: Confirms functional tetracycline resistance cassettes in plasmids, BACs, or cosmids by growth on selective LB agar.

Microbial Contaminant Exclusion in Mixed Cultures: Enriches for intended tet-resistant bacterial populations (e.g., E. coli) while suppressing susceptible contaminants.