Antibiotic Selection of Transformants: Selects Escherichia coli colonies carrying kanamycin-resistance plasmids after transformation.

Colony Screening: Isolates single colonies for downstream analyses such as colony PCR, sequencing, or miniprep validation.

Plasmid Maintenance: Maintains Kan^R plasmids/strains by routine streaking and passaging under selection.

Conjugation/Transposition Selection: Recovers transconjugants or transposon insertions bearing kanamycin markers in Escherichia coli or Agrobacterium tumefaciens.

Library Construction and Screening: Selects pooled libraries (e.g., CRISPRi, transposon, expression libraries) with Kan^R markers.

CFU Enumeration Under Selection: Quantifies viable Kan^R cells from transformations or selections.

Single-Colony Isolation for Starter Cultures: Provides clonal isolates to initiate liquid cultures retaining Kan^R plasmids.

Genome Editing Selection: Screens recombineering or CRISPR edits that introduce kanamycin resistance cassettes in Escherichia coli.

Phenotypic Verification: Confirms maintenance of kanamycin resistance and plasmid stability across passages.

Agro-Mediated Workflows: Selects Kan^R plasmids in Agrobacterium tumefaciens for plant transformation pipelines.