Selection of Kanamycin-Resistant Transformants: Plating transformed E. coli and other kanR-bearing bacteria to isolate antibiotic-resistant colonies.

Plasmid Maintenance: Stable propagation of kanamycin-marked plasmids in E. coli, Pseudomonas putida, and other susceptible hosts.

Colony Screening: Picking single colonies for downstream verification (e.g., colony PCR or insert checks) from kanamycin-selective plates.

Transformation Efficiency and CFU Enumeration: Quantifying recovery and viability of kanamycin-resistant cells after transformation.

Conjugation/Transposition Selection: Selecting transconjugants or transposon insertions carrying kanamycin resistance in mixed populations.

Strain Preservation and Isolation: Streaking and short-term storage of kan-resistant strains to maintain clonal purity.

Starter Colony Preparation: Generating single-colony isolates for liquid culture inoculation and plasmid miniprep workflows.

Contamination Control: Suppressing growth of non-resistant contaminants while maintaining desired kanR strains such as Escherichia coli lab clones.