Antibiotic Selection of Transformants: Isolates kanamycin-resistant colonies after transformation of E. coli or other bacteria with kanR plasmids (30 µg/mL Kanamycin Sulfate).

Plasmid Maintenance and Propagation: Maintains selection for kanamycin-marked plasmids during routine streaking and passaging of E. coli.

Colony Screening for Cloning: Enables picking colonies for PCR, restriction analysis, and sequencing of kanR constructs.

Protein Expression Workflows: Selects kanR expression vectors in strains like E. coli BL21(DE3) prior to induction.

Conjugation and Mating Assays: Selects kanamycin-marked transconjugants in systems such as Agrobacterium tumefaciens–E. coli or Pseudomonas aeruginosa.

CRISPR/Gene Knockout Selection: Screens for insertions or replacements using kanamycin resistance cassettes in E. coli and other bacteria.

Transposon Mutagenesis: Selects kanR transposon insertions in organisms including E. coli and Bacillus subtilis.

Colony-Forming Unit (CFU) Enumeration: Quantifies viable kanamycin-resistant bacteria under defined selection pressure.

Strain Verification and Stability Checks: Confirms retention of kanamycin markers by re-streaking and phenotype validation.

Library Maintenance: Preserves plasmid or mutant libraries carrying kanR during storage and routine handling.