Selection of Kanamycin-Resistant Transformants: Plate transformed E. coli carrying KanR plasmids to isolate successful clones.

Plasmid Maintenance: Maintain and passage KanR plasmid-bearing E. coli strains under antibiotic selection.

Transformation Plating and CFU Enumeration: Quantify transformation efficiency by counting colonies on kanamycin-selective plates.

Colony Screening: Perform colony PCR or blue-white screening (with X-gal/IPTG) to identify correct KanR clones.

Molecular Cloning Workflows: Plate Gibson, Golden Gate, or ligation products on LB-Kan to select assembled constructs.

Protein Expression Strain Selection: Select recombinant E. coli (e.g., BL21 derivatives) harboring KanR expression vectors.

CRISPR/Cas Genome Editing: Select E. coli edits using kanamycin-marked cassettes or helper plasmids.

Transposon Mutagenesis Screens: Recover insertions marked with KanR in E. coli for downstream mapping.

Conjugation and Plasmid Transfer: Select recipients acquiring kanamycin-marked plasmids after mating.

Library Maintenance and Picking: Preserve and isolate clones from KanR plasmid libraries for glycerol stock preparation.