Antibiotic Selection of Transformants: Selects chloramphenicol-resistant colonies (50µg/mL) after transformation of E. coli or Agrobacterium tumefaciens with cat-marked plasmids.

Plasmid Maintenance: Maintains selection for cat-bearing plasmids in E. coli and Bacillus subtilis during routine streaking and passaging.

Clone Screening (Colony PCR/Sequencing): Enables picking chloramphenicol-resistant colonies for colony PCR, restriction analysis, and sequencing validation.

Library Construction and Screening: Supports BAC/fosmid/cosmid and genomic/cDNA library workflows using chloramphenicol resistance markers.

Transformation Efficiency and CFU Enumeration: Quantifies transformants by plating dilution series on selective media.

Strain Storage and Stability Under Selection: Preserves recombinant strains on plates to prevent plasmid loss during short-term storage.

Conjugation/Mating Selections: Recovers transconjugants carrying cat-marked elements in systems involving E. coli and Agrobacterium tumefaciens.

Protein Expression Workflows: Maintains cat-selectable expression vectors in E. coli for small-scale expression screens.