Antibiotic Selection of Transformants: Isolates chloramphenicol-resistant colonies after transformation of Escherichia coli (E. coli).

Plasmid Maintenance and Propagation: Maintains selection for cat-marked plasmids, BACs, cosmids, or fosmids in E. coli.

Clone Screening and Verification: Enables screening of recombinant libraries and verification of inserts by colony picking under chloramphenicol selection.

CFU Enumeration and Titering: Quantifies viable chloramphenicol-resistant cells for transformation efficiency and strain viability assessments.

Conjugation and Transposition Selection: Recovers transconjugants or transposon insertions carrying chloramphenicol resistance in E. coli or Bacillus subtilis.

Expression Strain Maintenance: Supports growth of strains harboring chloramphenicol-selected expression vectors to retain plasmid integrity.

Genome Editing Marker Selection: Selects for edits using chloramphenicol resistance cassettes in bacterial engineering workflows.

Streaking for Isolation and Short-Term Storage: Produces single colonies and preserves selective pressure during routine culture maintenance.