Selective Plating of Transformants: Recovery of colonies carrying chloramphenicol-resistant plasmids (e.g., cat-marked vectors, BACs, cosmids) in Escherichia coli.

Plasmid Maintenance: Routine culture and passaging to retain chloramphenicol-selectable plasmids in Escherichia coli expression and cloning strains.

Cloning and Library Screening: Colony isolation and picking during molecular cloning and library construction under chloramphenicol selection.

Streaking for Single Colonies: Strain purification and isolation of single colonies while maintaining chloramphenicol selection.

CFU Enumeration: Determination of viable cell counts for chloramphenicol-resistant cultures.

Replica Plating: Verification of chloramphenicol resistance or plasmid presence across multiple plates.

Conjugation/Mating Selections: Post-mating selection of chloramphenicol-resistant transconjugants.

Genome Editing Selections: Screening of clones in CRISPR/recombineering workflows when cat is used as the selectable marker.

Protein Expression Workflows: Maintenance of strains harboring chloramphenicol-selectable expression vectors prior to induction.

Quality Control and Strain Verification: Selective plating to confirm genotype, antibiotic resistance, and detect contamination.