Antibiotic Selection of Transformants: Isolates chloramphenicol-resistant colonies after transformation of Escherichia coli with cat-marked plasmids (10 µg/mL chloramphenicol).

Plasmid Maintenance: Maintains selection pressure for cat-bearing plasmids in Escherichia coli and other lab strains during routine passaging.

Cloning and Subcloning Workflows: Enables selection of correct recombinant clones carrying chloramphenicol resistance for downstream analyses.

Colony Isolation and CFU Enumeration: Streaks for single colonies and quantifies viable chloramphenicol-resistant cells from mixed cultures.

Protein Expression Systems: Supports selection of expression or helper plasmids with cat markers in co-expression strains of Escherichia coli.

BAC/Fosmid/Cosmid Library Work: Selects and propagates large-insert vectors that commonly use chloramphenicol resistance.

Conjugation and Mating Selections: Selects transconjugants carrying cat-marked elements in donor/recipient crosses (e.g., Escherichia coli, Salmonella).

Strain Verification and Screening: Supports colony PCR, plasmid prep, and sequencing from selected chloramphenicol-resistant colonies.

Contamination Control: Suppresses chloramphenicol-sensitive background flora while recovering target resistant strains such as Escherichia coli or Bacillus subtilis.

Starter Culture Preparation and Archiving: Picks single resistant colonies for liquid culture inoculation and glycerol stock creation.