Antibiotic Selection of Plasmid-Containing Escherichia coli: Selects for β-lactamase–encoded resistance using 150 µg/mL carbenicillin for robust plasmid maintenance.

Molecular Cloning Workflows: Plating transformants after ligation or Gibson assembly to recover recombinant clones.

Blue-White Screening (with X-gal/IPTG): Distinguishes insert-containing colonies on LB-carbenicillin when α-complementation vectors are used.

Colony PCR and Sanger Prep: Picking colonies for rapid insert verification directly from selective plates.

Transformation Efficiency and CFU Enumeration: Quantifying transformants and optimizing competent cell performance under carbenicillin selection.

Protein Expression Strain Selection: Maintaining expression plasmids in T7-based or other E. coli systems prior to induction.

CRISPR/Cas Genome Editing Workflows: Selecting cells carrying editing or helper plasmids conferring carbenicillin resistance.

Bacterial Conjugation/Plasmid Transfer Selection: Selecting transconjugants when the mobilized plasmid carries a carbenicillin resistance marker.

Glycerol Stock Preparation: Isolating single colonies for long-term storage of carbenicillin-resistant E. coli strains.

Routine Plasmid Maintenance: Preserving low- to high-copy vectors in laboratory E. coli strains under consistent β-lactam selection.