Antibiotic Selection of Plasmids: Selects and maintains beta-lactam resistance plasmids (AmpR/CarbR) in Escherichia coli using 100 µg/mL carbenicillin.

Transformation Plating: Enables recovery and isolation of transformants after chemical or electroporation transformation of Escherichia coli.

Cloning and Colony Picking: Supports cloning workflows for colony isolation, propagation, and plasmid maintenance in Escherichia coli.

Blue-White Screening: Used for insert screening when supplemented with X-Gal/IPTG to differentiate recombinant clones on carbenicillin plates.

Colony PCR/Screening: Provides stable growth for picking colonies for PCR, restriction analysis, or sequencing verification.

CFU Enumeration: Quantifies transformation efficiency and bacterial titers by counting colony-forming units.

Strain Maintenance: Used for routine streaking, single-colony isolation, and preparing colonies for glycerol stock banking.

Protein Expression Workflows: Maintains expression strains carrying beta-lactam–selectable plasmids prior to liquid culture induction.

CRISPR/Genome Editing Plasmids: Selects and propagates editing vectors with beta-lactam resistance in Escherichia coli.

Satellite Colony Reduction: Preferred over ampicillin plates to minimize satellite colonies in high-density selections.