Selection of Transformants: Isolates carbenicillin-resistant Escherichia coli colonies after heat-shock or electroporation transformation of β-lactamase–encoded plasmids (75 µg/mL carbenicillin).

Clonal Isolation and Maintenance: Streaks for single colonies and maintains plasmid-bearing E. coli strains on LB agar (1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl, 1.5% Agar).

Colony Screening: Enables colony PCR, restriction analysis, or sequencing from selected E. coli clones carrying ampicillin/carbenicillin-resistance plasmids.

Blue-White Screening (with supplements): Supports α-complementation screening of E. coli when supplemented with X-gal/IPTG.

CFU Enumeration: Quantifies transformation efficiency by plating serial dilutions and counting colonies under carbenicillin selection.

Plasmid Stability Assays: Assesses segregational stability of β-lactamase–marked plasmids in E. coli under 75 µg/mL carbenicillin.

Library Plating: Plates combinatorial or genomic libraries in E. coli to ensure selectable coverage of transformants.

Replica Plating: Verifies antibiotic resistance markers and clone identity by transferring colonies onto selective media.

Strain Verification: Confirms plasmid presence/expression in engineered E. coli strains prior to liquid culture scale-up.

Glycerol Stock Recovery: Recovers and re-isolates clonal E. coli lines from frozen stocks under carbenicillin selection.