Plasmid Selection and Maintenance: Selects and maintains bla (β-lactamase)-bearing plasmids in Escherichia coli using 50 µg/mL carbenicillin.

Bacterial Transformation Recovery and Screening: Enables post-transformation growth and selection of recombinant E. coli colonies carrying carbenicillin-resistant plasmids.

Cloning Workflows (Ligation/Gibson/Golden Gate): Supports selection of correctly assembled constructs on solid LB medium.

Colony Isolation and Purification: Streaking for single colonies to derive clonal, antibiotic-selected strains.

Colony PCR and Mini-Prep Pre-Screening: Picking antibiotic-resistant colonies for rapid insert verification.

Blue-White Screening (with X-gal/IPTG): Used for alpha-complementation screening when supplemented with chromogenic inducers.

CFU Enumeration Under Selection: Quantifying transformation efficiency and viable counts of resistant E. coli on selective plates.

Pre-Expression Strain Qualification: Verifying plasmid carriage in protein expression strains prior to induction.

Routine Strain Storage and Propagation: Maintaining glycerol stock derivatives and working plates with selective pressure.

Reduced Satellites vs. Ampicillin: Preferentially used to minimize satellite colony formation during selection on LB agar.