Antibiotic Selection of Ampicillin-Resistant Transformants: Selects AmpR colonies after transformation of E. coli with plasmids carrying bla (AmpR).

Plasmid Cloning and Screening: Enables screening of recombinant plasmids by growing only AmpR E. coli colonies.

Blue-White Screening: Used with IPTG/X-Gal for lacZα-based blue-white screening on Amp plates in E. coli.

Strain Maintenance and Propagation: Maintains selection for AmpR plasmids during routine passage of E. coli cultures.

Colony Isolation and Picking: Streaks and isolates single AmpR colonies for downstream workflows.

Colony PCR and Sequencing Prep: Provides selective growth for picking colonies used in colony PCR or plasmid prep.

CFU Enumeration and Transformation Efficiency: Quantifies colony-forming units on selective medium to assess transformation efficiency.

Protein Expression Workflows: Selects and maintains expression strains harboring AmpR expression vectors in E. coli.

Conjugation/Plasmid Transfer Selection: Selects transconjugants carrying AmpR plasmids following mating or electroporation in E. coli.

Glycerol Stock Preparation: Preserves verified AmpR E. coli clones for long-term storage.