L0286

LB Agar with Puromycin-100, 100mm, 20 Plates per Sleeve, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

100mm

Item Count

20 Plate(s)

Price per Item

$219.00

Fill Volume

25 mL

LB Agar with Puromycin-100, 100mm, 20 Plates per Sleeve, Sterile.

See label

COA

L0286

LB Agar with Puromycin-100, 100mm, 20 Plates per Sleeve, Sterile.

Quantity

Minimum is set to 8 for this product*

scroll

Description

Formulation

Common Applications

Storage

LB Agar with Puromycin-100 is a nutrient agar medium that supports the growth and selection of puromycin-resistant bacterial populations. The matrix provides peptides, amino acids, vitamins, and appropriate ionic strength for routine culture, and agar supplies a firm surface for colony isolation.

Puromycin dihydrochloride at 100 µg/mL enables selection of cells expressing puromycin N-acetyltransferase (pac) by inhibiting protein synthesis in non-resistant cells.

1.0% Tryptone
0.5% Yeast Extract
1.0% Sodium Chloride
1.5% Agar
100µg/mL Puromycin Dihydrochloride

Selection of Puromycin-Resistant Clones: Isolating bacterial transformants carrying pac-encoded puromycin resistance on LB agar with 100 µg/mL puromycin.
Plasmid Maintenance in E. coli Hosts: Propagation of pac-containing plasmids in strains such as E. coli DH5α, TOP10, Stbl3, and BL21(DE3).
Colony Screening and Picking: Selecting recombinant colonies for cloning workflows under puromycin pressure.
Streaking for Single Colonies: Obtaining clonal isolates and ensuring plasmid stability with continuous selection.
CFU Enumeration: Quantifying transformation efficiency and viability of puromycin-resistant bacteria.
Glycerol Stock Preparation: Creating long-term stocks of validated puromycin-resistant clones.
Pre-Culture for Plasmid Prep: Growing selected clones for downstream miniprep/maxiprep of pac-bearing vectors.
Contamination Control: Suppressing growth of non-resistant contaminants while maintaining resistant strains.
Shuttle Vector Propagation: Bacterial amplification of plasmids destined for puromycin selection in mammalian cells.

Storage Temperature

Refrigerated

Shelf Life

90 days

Looking for a Certificate of Analysis?

Enter a product’s Lot Number to download the Certificate of Analysis (COA).

GET YOUR COA

A trusted partner that

can help you scale

With almost 30 years of experience manufacturing custom reagents, our rapid

turnaround of customer-specified products helps you scale from RUO to GMP.

Raising the bar on quality

We follow rigorous testing and documentation procedures that go beyond industry standards.

Delivery in weeks, not months

With our modular manufacturing system, we can get you custom solutions in weeks, not months.

Add our expertise to your team

Our scientists and engineers can help optimize a solution for your specific application.

We're custom by default

Everything we do is built around getting you exactly what you need, from formulation and filtration to packaging. Talk to our team about creating a custom product today.

Let's find your solution

Products

Categories

Content

LB Agar with Puromycin-100, 100mm, 20 Plates per Sleeve, Sterile.

Certificate of Analysis

Looking for a Certificate of Analysis?

A trusted partner that

can help you scale

Raising the bar on quality

Delivery in weeks, not months

Add our expertise to your team

We're custom by default

Making solutions possible