Bacterial Antibiotic Selection: Selects transformants carrying kanamycin resistance on plates and in liquid culture using 50mg/mL Kanamycin Sulfate stock for dilution.

Plasmid Maintenance and Expression: Maintains kanR plasmids during cloning and protein expression in Escherichia coli and other bacteria.

Agrobacterium Workflows: Supports selection and maintenance of kanR vectors in Agrobacterium tumefaciens for plant transformation protocols.

Plant Tissue Culture Selection: Selects nptII/neo-marked transgenics in callus and regeneration media after Agrobacterium-mediated transformation.

Yeast Genetics (KanMX): Enables selection of KanMX-marked integrations and deletions in Saccharomyces cerevisiae.

Bacterial Gene Knockouts/Transposon Mutagenesis: Selects kanamycin-resistant insertion mutants in bacteria such as Escherichia coli, Pseudomonas, and Bacillus subtilis.

Culture Contamination Control: Supplement cell culture media to suppress bacterial contaminants when appropriate laboratory guidelines allow.