Bacterial Selection in Cloning: Selects and maintains plasmids bearing kanamycin-resistance markers in Escherichia coli and other bacteria.

Protein Expression Workflows: Maintains selection pressure for expression vectors during protein production in Escherichia coli.

Microbiology Media Supplementation: Added to agar and broth to isolate and propagate kanamycin-resistant strains such as Escherichia coli and Bacillus subtilis.

Antimicrobial Susceptibility Testing: Used in MIC and disk diffusion assays to evaluate bacterial sensitivity, including Gram-negative organisms like Pseudomonas aeruginosa.

Cell Culture Contamination Control: Suppresses bacterial contaminants in mammalian cell culture as an alternative or adjunct to other antibiotics.

Plant Transformation Selection: Selects nptII (kanR) transgenic plant tissues following Agrobacterium tumefaciens-mediated transformation.

Algal and Cyanobacterial Selection: Enables maintenance of kanamycin-resistant constructs in photosynthetic microbes.

Fermentation and Bioprocessing: Maintains plasmid stability and minimizes bacterial contamination during microbial fermentation runs.

Strain Banking and Storage: Preserves selection for kanamycin-resistant strains in glycerol stocks and seed cultures.

Quality Control in Manufacturing: Employed in USP-compliant microbiological workflows and media qualification where Kanamycin Sulfate, USP Grade is specified.