Antibiotic Selection in Bacteria: Maintains plasmids carrying kanamycin-resistance (nptII/aphA) in E. coli and other susceptible bacteria during cloning and culture.

Recombinant Protein Expression: Ensures selection of expression vectors in E. coli for consistent protein production in shake flasks and bioreactors.

Bacterial Media Supplementation: Added to LB, TB, or minimal media agar/broth to select and maintain kanamycin-resistant colonies.

Plant Transformation Selection: Selects transgenic plant cells expressing nptII in tissue culture and regeneration workflows, including Agrobacterium-mediated protocols.

Cell Culture Contamination Control: Helps suppress growth of susceptible bacteria in mammalian and insect cell cultures.

Microbial Susceptibility Testing: Used to determine MICs and perform antimicrobial susceptibility assays for quality control and research.

Strain and Plasmid Maintenance: Preserves selection pressure in starter cultures, glycerol stocks, and long-term storage of engineered bacterial lines.