Antibiotic Selection in Bacteria: Supplement LB agar/broth (typically 25–50 µg/mL) to select and maintain plasmids carrying kanamycin resistance in E. coli and other Gram-negative bacteria.

Plasmid Maintenance for Cloning: Ensure retention of kanamycin-resistant cloning vectors during routine propagation in E. coli.

Protein Expression Cultures: Maintain selection pressure on expression plasmids in E. coli during starter and induction cultures.

Bacterial Library and Mutagenesis Screens: Select transposon or insertion libraries using kanamycin resistance markers in E. coli and related hosts.

Conjugation and Strain Construction: Select donor/recipient strains and recombinant constructs after mating or recombineering in E. coli.

Maintenance of Large Vectors: Support selection of BACs, fosmids, and cosmids bearing kanamycin resistance in E. coli.

CRISPR/Cas Editing Workflows in Bacteria: Select editing plasmids or helper constructs with kanamycin resistance in E. coli.

Plant Transformation Selection: Select transgenic plant tissues expressing nptII (kanamycin resistance) in tissue culture following Agrobacterium-mediated transformation.

Cyanobacteria/Algal Selection: Apply kanamycin as a selectable marker in engineered cyanobacteria and microalgae.

Agrobacterium Workflows: Maintain plasmids bearing kanamycin resistance in Agrobacterium tumefaciens for plant transformation vectors.