25mM Histidine, pH 6.0 is a ready-to-use histidine buffer in purified water, adjusted to pH 6.0. Histidine buffers effectively near its pKa of about 6.0. The 1 L fill supports laboratory and pilot-scale diafiltration.

It is commonly used as a formulation, holding, and diafiltration buffer for proteins and biologics, including antibodies and ADCs, in the mildly acidic range where histidine buffers well.