Protein Formulation and Stabilization: 25mM Histidine Buffer at pH 6.0 is commonly used to stabilize proteins and monoclonal antibodies during storage and handling.

Cation Exchange Chromatography: Employed as an equilibration, wash, or elution buffer to maintain pH 6.0 conditions for protein binding and separation.

Size-Exclusion Chromatography (SEC): Used as a mobile phase to preserve protein conformation and minimize aggregation during SEC.

Enzyme Assays: Provides a consistent pH 6.0 environment to support enzymatic activity profiles that require mildly acidic conditions.

Dialysis and Buffer Exchange: Serves as the target buffer to transition samples into a stable 25mM Histidine Buffer system.

Ultrafiltration/Diafiltration (UF/DF): Used as diafiltration buffer for concentrating and exchanging proteins into a histidine-based matrix.

Biologic and Vaccine Formulations: Included as a buffering excipient to maintain product stability and pH during formulation and fill-finish.

Lyophilization Buffers: Provides pH control and stabilization for proteins during freeze-drying and reconstitution.

Analytical Sample Preparation: Maintains defined pH conditions for reproducible HPLC, LC-MS, and spectroscopic analyses.