Antibody Elution (Protein A/G/L Chromatography): Elutes bound IgG with low-pH conditions using 0.1M Glycine, pH 2.7, followed by rapid neutralization.

Immunoprecipitation (IP) Elution: Releases antigen–antibody complexes from beads with 0.1M Glycine, pH 2.7, preserving target integrity.

Western Blot Antibody Stripping: Removes primary/secondary antibodies from membranes using acidic 0.1M Glycine for reprobing.

Formaldehyde Crosslink Quenching (ChIP/Crosslinking): Stops fixation reactions by quenching reactive aldehydes with 0.1M Glycine.

NHS-Ester Labeling Quench: Terminates amine-reactive (NHS) conjugation reactions and reduces background using 0.1M Glycine.

Ligand–Receptor Dissociation Assays: Employs transient low pH (0.1M Glycine, pH 2.7) to dissociate surface-bound complexes for recycling studies.

Phage Display/Biopanning Elution: Uses low-pH 0.1M Glycine to elute bound phage from targets during selection cycles.

Protein Purification Cleanup: Removes nonspecific proteins from affinity matrices via brief acidic washes with 0.1M Glycine.

Sample Neutralization/Conditioning Steps: Provides controlled pH shifts before immediate neutralization in workflows requiring transient acidification.

Membrane and Bead Regeneration: Applies low pH (0.1M Glycine, pH 2.7) to regenerate affinity media between runs.