G4510

1M Glycine Buffer, pH 3.2. 1000mL, Sterile

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$46.00

Fill Volume

1000 mL

See label

COA

G4510

1M Glycine Buffer, pH 3.2. 1000mL, Sterile

Quantity

Minimum is set to 2 for this product*

scroll

Description

Formulation

Common Applications

Quality Specifications

Storage

1 M Glycine Buffer, pH 3.2, is an acidic, zwitterionic buffer suitable for procedures requiring low pH conditions. The solution maintains pH near the carboxyl pKa of glycine and provides high ionic strength for controlled pH adjustment in biochemical workflows.

Applications include elution of antibodies from Protein A/G affinity matrices, dissociation of immune complexes, stripping of proteins from solid supports, pH-shift elution in chromatography, sample acidification followed by neutralization, and pH control in electrophoresis and enzyme inactivation steps.

1M Glycine

Protein A/G Affinity Chromatography Elution: Acidic elution of antibodies and Fc-fusion proteins; commonly diluted from 1M to 0.1M glycine, pH ~2.7–3.2, then neutralized.
Western Blot/Membrane Reprobing: Elution of bound primary/secondary antibodies from membranes to enable reprobing.
Immune Complex Dissociation: Acidic dissociation of antigen–antibody complexes prior to analysis (e.g., ELISA, immunoprecipitation).
Cell Surface Antibody Stripping: Rapid removal of surface-bound antibodies from live cells for receptor internalization/recycling assays.
Phage Display Selections: Acid elution of target-bound phage particles during panning cycles.
Low-pH Triggering/Inactivation Studies: Rapid acidification for virus fusion assays and low-pH inactivation experiments.
Protein–Protein Interaction Disruption: Acid-mediated dissociation of weak interactions for downstream purification or analysis.

pH: 3.20 ± 0.04

Storage Temperature

Room Temperature

Shelf Life

1095 days

Looking for a Certificate of Analysis?

Enter a product’s Lot Number to download the Certificate of Analysis (COA).

GET YOUR COA

A trusted partner that

can help you scale

With almost 30 years of experience manufacturing custom reagents, our rapid

turnaround of customer-specified products helps you scale from RUO to GMP.

Raising the bar on quality

We follow rigorous testing and documentation procedures that go beyond industry standards.

Delivery in weeks, not months

With our modular manufacturing system, we can get you custom solutions in weeks, not months.

Add our expertise to your team

Our scientists and engineers can help optimize a solution for your specific application.

We're custom by default

Everything we do is built around getting you exactly what you need, from formulation and filtration to packaging. Talk to our team about creating a custom product today.

Let's find your solution

Products

Categories

Content

1M Glycine Buffer, pH 3.2. 1000mL, Sterile

Certificate of Analysis

Looking for a Certificate of Analysis?

A trusted partner that

can help you scale

Raising the bar on quality

Delivery in weeks, not months

Add our expertise to your team

We're custom by default

Making solutions possible