Nucleic Acid Extraction (Silica-Based): 6M Guanidine Hydrochloride acts as a chaotropic agent to lyse samples, inactivate nucleases, and promote DNA/RNA binding to silica matrices.

RNA Preservation: Maintains RNase-inactivating conditions during tissue and cell homogenization for downstream RT-qPCR and sequencing.

Protein Denaturation and Refolding: Solubilizes inclusion bodies from E. coli and supports unfolding/refolding screens for protein recovery.

Denaturing IMAC Purification: Enables His-tagged protein purification under denaturing conditions to recover insoluble or aggregated targets.

Sample Lysis for Molecular Diagnostics: Provides chaotropic lysis and enzyme inactivation for downstream PCR/RT-qPCR workflows.

Chromatography Under Denaturing Conditions: Facilitates HIC elution and SEC runs requiring complete protein unfolding.

Prevention of Protein Aggregation: Disrupts intermolecular interactions to dissolve aggregates during sample preparation.

Nucleic Acid Hybridization Stringency: Increases stringency in certain hybridization and wash buffers for probe-based assays.

Protein Stability and Unfolding Studies: Used to generate chemical denaturation curves for thermodynamic stability measurements.