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F3062

10X Ficoll Loading Buffer. 20mL, Non-Sterile.

Availability

Ships in 6 - 14 Days

Container Size

20mL

Item Count

1 Bottle(s)

Price per Item

$110.00

Fill Volume

20 mL

F3062

10X Ficoll Loading Buffer. 20mL, Non-Sterile.

Quantity

Minimum is set to 25 for this product*

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Description
Formulation
Common Applications
Storage

10X Ficoll Loading Buffer, Non-Sterile is a concentrated sample loading solution for gel electrophoresis workflows. The high-density matrix facilitates clean sample deposition into gel wells, a chelating agent helps protect nucleic acids from divalent cation–dependent nucleases, an anionic surfactant promotes uniform sample migration, and dual tracking dyes provide visual monitoring of electrophoretic progress.

This buffer is supplied at 10X concentration and is typically diluted to 1X prior to use. The solution is visually blue due to the tracking dyes. Non-sterile; intended for laboratory research applications.

  • 20.00% Ficoll
  • 100mM Ethylenediaminetetraacetic acid (EDTA)
  • 1.00% SDS
  • 0.25% Xylene Cyanol
  • 0.25% Bromophenol Blue
  • Agarose Gel Electrophoresis Sample Loading (DNA/RNA): 20.00% Ficoll increases sample density for clean well loading; 0.25% Xylene Cyanol and 0.25% Bromophenol Blue provide migration tracking.

  • PCR Product Analysis: Mix with amplicons before loading to visualize and track fragments during electrophoresis.

  • Restriction/Ligation Reaction Quenching: 100 mM EDTA chelates Mg2+ to stop enzyme activity; 1.00% SDS denatures proteins for direct gel loading.

  • Nuclease Inhibition During Sample Prep: EDTA protects DNA/RNA from degradation prior to and during gel loading.

  • Tracking Dye Reference for Run Progress: Xylene Cyanol and Bromophenol Blue indicate approximate migration fronts for size estimation and run timing.

  • Loading of Plasmid/Genomic DNA from Crude Extracts: SDS aids release of nucleic acids from protein complexes for quick screening (e.g., colony PCR from Escherichia coli).

  • RNA Sample Loading for Northern-Style Agarose Gels: Provides density and tracking dyes while EDTA limits RNase activity.

  • General Sample Preparation for TAE/TBE Gels: Compatible with common agarose electrophoresis systems for nucleic acid analysis.

Storage Temperature
Frozen
Shelf Life
730 days
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