Plasmid Selection in E. coli: Selects for bla (β-lactamase)–encoded ampicillin/carbenicillin resistance in cloning and routine propagation.

Maintenance of Expression Constructs: Preserves plasmid-bearing E. coli in liquid cultures and agar plates during protein expression and strain storage.

Cloning and Transformation Workflows: Used post-transformation for recovery and selective plating of recombinant colonies.

Blue-White Screening: Supports selection and screening on X-gal/IPTG plates for insert verification in E. coli.

Reduced Satellite Colonies: Preferred over ampicillin to minimize satellite colony formation on plates.

Media Supplementation: Added to LB/TB/agar to prepare carbenicillin-selective media at standard working concentrations.

Protein Production in E. coli: Maintains plasmid selection during small- to large-scale expression cultures.

Plant Tissue Culture Support: Applied to eliminate residual Agrobacterium tumefaciens after transformation and reduce bacterial contamination.