Plasmid Selection in Escherichia coli: Selects for bla (ampicillin/carbenicillin-resistance) plasmids after transformation on plates and in liquid culture.

Plasmid Maintenance During Protein Expression: Maintains selection for beta-lactamase–bearing vectors in recombinant protein production in E. coli.

Agar Plate Selection with Reduced Satellites: Preferred over ampicillin to minimize satellite colony formation during cloning.

Selective Media Preparation: Added to LB/agar to a typical working concentration of 50–100 µg/mL from a 100 mg/mL stock for routine bacterial selection.

Plasmid Amplification for DNA Prep: Ensures retention of expression and cloning vectors in E. coli for mini-/maxipreps.

Selection in Gram-Negative Bacteria: Effective against susceptible species including Pseudomonas aeruginosa for beta-lactamase–based selection.

Plant Tissue Culture (Post-Transformation Cleanup): Used to suppress or eliminate residual Agrobacterium tumefaciens after plant transformation.