Antibiotic Selection in Bacterial Cloning: Selects for chloramphenicol-resistance (cat) markers in Escherichia coli, Bacillus subtilis, and related hosts on agar plates and in liquid culture.

Plasmid Maintenance: Maintains chloramphenicol-resistant plasmids and expression constructs during routine propagation in Escherichia coli and other bacteria.

BAC/Fosmid Workflows: Used to select and maintain bacterial artificial chromosomes and fosmids carrying cat markers in Escherichia coli.

Plasmid Copy-Number Amplification: Employed to amplify ColE1-type plasmid copy number in Escherichia coli prior to DNA purification.

Genetic Engineering and Recombineering: Provides selection for cat cassettes in allelic exchange, transposon mutagenesis, and genome editing workflows in Escherichia coli and Bacillus subtilis.

Shuttle Vector Selection in Gram-positives: Enables selection of cat-marked plasmids and chromosomal integrations in Staphylococcus aureus and Bacillus subtilis.

Protein Synthesis Inhibition Assays: Inhibits bacterial translation for studies such as ribosome profiling, translation arrest, and antibiotic sensitivity testing in Escherichia coli and other bacteria.

Agrobacterium Workflows: Selection and maintenance of cat-marked constructs in Agrobacterium tumefaciens used for plant transformation protocols.