Antibiotic Selection in Cloning/Transformation: Selects Escherichia coli/E. coli carrying bla/AmpR plasmids at 50–100 µg/mL, reducing satellite colonies compared to ampicillin.

Plasmid Maintenance in Liquid Culture: Maintains selection pressure in overnight and preparative cultures of E. coli for stable plasmid retention.

Agar Plate and Media Preparation: Supplementation of LB or other media for plate pouring and liquid growth at standard working concentrations.

Protein Expression Cultures: Supports selection of AmpR expression vectors in E. coli during induction and scale-up.

Blue-White Screening: Used on IPTG/X-Gal plates to select and screen recombinant colonies in E. coli.

Library Construction and Colony Picking: Enables selection of large plasmid libraries and robust colony isolation in E. coli.

Glycerol Stock Preservation: Maintains selective pressure during preparation and storage of E. coli stocks.

Plant Tissue Culture (Post-Transformation): Helps eliminate Agrobacterium tumefaciens overgrowth after co-cultivation.

Control of Gram-Negative Contaminants: Active against organisms such as Pseudomonas aeruginosa in microbial workflows.