Plasmid Selection in Escherichia coli (E. coli): Alternative to ampicillin for selecting bla-bearing plasmids; more stable in media and reduces satellite colonies.

Maintenance of Plasmids in Liquid and Solid Media: Used at typical working concentrations of 50–100 µg/mL from a 100 mg/mL stock to maintain selective pressure during growth and protein expression.

Transformation and Cloning Workflows: Enables selection of transformants on LB agar or in broth, improving colony discrimination compared to ampicillin.

Selection in Non-Enteric Gram-Negatives: Employed for selection in organisms such as Pseudomonas aeruginosa where carbenicillin provides effective beta-lactam selection.

Plant Tissue Culture (Post-Transformation Cleanup): Used to suppress or eliminate Agrobacterium tumefaciens after cocultivation while regenerating plant tissues.

Preparation of Antibiotic Plates and Media: Added to cooled sterile media to create selective LB/TB plates and broths for routine bacterial work.

Stability-Sensitive Applications: Preferred where reduced beta-lactam hydrolysis and fewer satellite colonies are critical for reliable selection.