Antibiotic Selection in Cloning: Selects for beta-lactam–resistant transformants carrying bla/ampR plasmids in Escherichia coli, with fewer satellite colonies than ampicillin.

Plasmid Maintenance in Liquid Culture: Sustains selection pressure for recombinant plasmids during overnight growth and protein expression in E. coli.

Preparation of Selective Media: Added to LB or other media and agar plates for routine selection and screening of transformants.

Blue-White Screening Support: Provides antibiotic selection in conjunction with X-gal/IPTG for lacZ-based colony screening.

Reduced Contamination and Background: Inhibits growth of beta-lactam–sensitive bacteria in cultures and on plates.

High-Expression Workflows: Maintains plasmids during induction for recombinant protein production in E. coli.

Plant Transformation Workflows: Suppresses/eliminates residual Agrobacterium tumefaciens after plant tissue transformation and co-cultivation.