Plasmid Selection and Maintenance in Escherichia coli: Selects for bla (β-lactamase) plasmids in cloning and protein expression workflows using a 100 mg/mL sterile stock for 50–100 µg/mL working concentrations.

Selective LB/Agar Plates: Added post-sterilization to prepare plates that reduce satellite colonies compared to ampicillin, improving colony picking accuracy.

Ampicillin Replacement: Used as a more stable β-lactam alternative in media, sustaining effective selection during long incubations.

Library and Screening Workflows: Enables reliable antibiotic selection in cloning libraries, bacterial two-hybrid, and high-throughput screens in E. coli.

Fermentation and Scale-Up: Maintains plasmid-bearing E. coli cultures in bioreactors for recombinant protein or DNA production.

Plant Transformation Workflows: Helps suppress or eliminate Agrobacterium tumefaciens after co-cultivation, supporting plant tissue culture cleanup.

Control of Gram-Negative Contaminants: Broad activity against organisms like Pseudomonas aeruginosa to limit contamination in microbial workflows.