Bacterial Plasmid Selection: Selects bla-bearing transformants in Escherichia coli during cloning and subcloning.

Protein Expression in E. coli: Maintains beta-lactamase–encoded plasmids in recombinant Escherichia coli expression strains.

Selective Media Preparation: Added to LB/agar to create selection plates for beta-lactam–resistant colonies.

Reduced Satellite Colonies: Used as a more stable alternative to ampicillin to minimize satellite colony formation in blue-white screening.

Plant Tissue Culture: Eliminates residual Agrobacterium tumefaciens after transformation and supports regeneration steps.

Contamination Control: Suppresses Gram-negative contaminants such as Pseudomonas aeruginosa in mixed cultures.

Cell Culture Antibiotic: Employed in non-therapeutic applications to reduce bacterial contamination in media.