Plasmid Selection in Escherichia coli (bla/AmpR): Selects for bacteria harboring beta-lactamase–encoded plasmids on plates and in liquid culture.

Plasmid Maintenance: Maintains selection pressure for bla-marked plasmids during overnight growth and scale-up.

Cloning Screens: Reduces satellite colony formation versus ampicillin during blue/white and colony screening.

Protein Expression Workflows: Ensures retention of expression plasmids in Escherichia coli during induction and expression.

Selection in Pseudomonas aeruginosa and other Gram-negatives: Provides improved activity where ampicillin is less effective.

Plant Transformation/Tissue Culture: Eliminates residual Agrobacterium tumefaciens after co-cultivation and during regeneration.

Bacterial Conjugation/Mating: Selects for transconjugants carrying bla-marked plasmids while suppressing donor strains.

Antimicrobial Media Supplement: Inhibits beta-lactam–susceptible contaminants in routine bacterial culture and plating.