Plasmid Selection in Escherichia coli: Selects and maintains bla-bearing plasmids in liquid and agar media for cloning and expression.

Ampicillin Alternative: More stable in culture media than ampicillin, reducing satellite colony formation during bacterial selection.

Fermentation and Scale-Up: Maintains plasmid-bearing E. coli populations during high-density growth for recombinant protein or DNA production.

Plant Transformation Workflows: Eliminates residual Agrobacterium tumefaciens after transformation and during callus/shoot regeneration.

Contamination Control in Cell Culture: Suppresses Gram-negative bacterial contaminants in mammalian and insect cell culture media.

Selection in Other Gram-Negatives: Used with Pseudomonas aeruginosa and related species carrying beta-lactamase plasmids.

Routine Cloning and Screening: Provides reliable beta-lactam selection for subcloning, library construction, and colony screening.