Bacterial Plasmid Selection and Maintenance: Selects and maintains chloramphenicol-resistant plasmids in E. coli and other bacteria on agar plates and in liquid culture.

BAC/Cosmid Library Workflows: Maintains bacterial artificial chromosomes and cosmids carrying cat markers in E. coli.

Recombinant Protein Expression: Enables co-selection of helper or expression plasmids with chloramphenicol resistance (e.g., pLysS/pLysE-Cam) in E. coli.

Phage Display and Two-Hybrid Systems: Provides antibiotic selection for chloramphenicol-marked vectors in bacterial display and interaction assays.

Translation Inhibition Studies: Inhibits bacterial protein synthesis for ribosome profiling and translation arrest experiments in organisms such as E. coli and Bacillus subtilis.

Gram-Positive Bacterial Selection: Supports selection of chloramphenicol-resistant plasmids in species like Bacillus subtilis and Staphylococcus aureus.

Agrobacterium Vector Maintenance: Maintains chloramphenicol-selectable binary vectors in Agrobacterium tumefaciens for plant transformation workflows.

Contamination Control: Temporarily suppresses bacterial contamination in eukaryotic and insect cell cultures when needed.