Bacterial Plasmid Selection and Maintenance: Selects and maintains chloramphenicol-resistant plasmids in Escherichia coli (E. coli) and other bacteria.

BAC/Cosmid/PAC Workflows: Supports selection and stable maintenance of large-capacity cloning vectors in E. coli.

Protein Expression Workflows: Ensures retention of chloramphenicol-selectable expression vectors during recombinant protein production in E. coli.

Agrobacterium and Plant Biology: Used for selection of chloramphenicol-marked plasmids in Agrobacterium tumefaciens during plant transformation workflows.

Gram-Positive Systems: Selection and maintenance of chloramphenicol-resistant constructs in organisms such as Bacillus subtilis.

Microbial Contamination Control in Fungal/Yeast Media: Added to culture media to suppress bacterial growth during isolation or propagation of fungi and yeasts.

Chloramphenicol Acetyltransferase (CAT) Assays: Substrate for CAT reporter gene assays and enzymatic activity studies.

Ribosome and Translation Research: Tool compound to inhibit prokaryotic protein synthesis for mechanistic studies.

Antibiotic Susceptibility and QC Studies: Reference antibiotic in microbiology testing and quality control workflows.

Mixed-Culture Selection: Enrichment of chloramphenicol-resistant strains within mixed bacterial populations, including Staphylococcus aureus and Salmonella enterica, for genetic studies.