Plasmid Selection in Bacteria: Selects and maintains cat-marked plasmids in E. coli and other susceptible bacteria using chloramphenicol resistance.

Co-Selection for Multi-Plasmid Systems: Enables simultaneous maintenance of multiple vectors (e.g., chloramphenicol + ampicillin/kanamycin) in recombinant E. coli protein expression workflows.

BAC/Fosmid Maintenance: Preserves chloramphenicol-resistant BACs/fosmids in E. coli during cloning and recombineering.

Helper Plasmid Retention in T7 Systems: Maintains chloramphenicol-resistant helper plasmids (e.g., pLysS/pLysE) in protein expression strains of E. coli.

Antimicrobial Susceptibility Testing: Serves as a reference antibiotic for MIC and inhibition assays against organisms such as E. coli, Salmonella, and Staphylococcus.

Contamination Control: Suppresses bacterial contaminants in eukaryotic cell and yeast cultures when used at appropriate dilutions.

Protein Synthesis Inhibition Studies: Blocks prokaryotic translation for mechanistic studies and ribosome profiling in bacteria (e.g., E. coli).

Mitochondrial/Chloroplast Translation Inhibition: Inhibits organellar protein synthesis in mammalian and plant cells to dissect compartment-specific translation.