Anaerobic Isolation and Cultivation: Supports growth of Clostridium spp. from clinical, food, and environmental samples using a reducing formulation (0.06% Cysteine-HCl) and rich nutrients (peptones, extracts, 0.50% Glucose).

Enumeration of Anaerobes: Used to count clostridia and mixed anaerobic flora on plates with consistent pH 6.8 and 2.10% Agar for colony isolation.

Spore Recovery After Stress: Facilitates germination and recovery of heat- or chemically-stressed clostridial spores with 0.50% Sodium Acetate and 0.10% Starch supporting repair and growth.

Food and Dairy Testing: Employed for detection and enumeration of sulfite-reducing clostridia, including Clostridium perfringens, in process and finished products.

Pre-Enrichment Prior to Selective Media: Non-selective recovery medium before plating to selective/differential media for Clostridium spp., including Clostridioides difficile.

Maintenance and Subculture of Anaerobic Stocks: Used to maintain viability and purity of reference and working cultures of Clostridium spp.

Environmental Monitoring for Anaerobic Contaminants: Plates used in monitoring programs to detect clostridial contamination on surfaces, air (settle plates), and water concentrates.

Fermentation and Metabolic Studies: Suitable for assessing colony morphology and basic fermentation traits in clostridia (e.g., Clostridium acetobutylicum).