Bacterial Selection and Plasmid Maintenance: Selects for AmpR plasmids in Escherichia coli and other susceptible bacteria during cloning and routine culture.

Transformation Screening on Agar Plates: Enables selection of transformants on LB agar; compatible with blue-white screening (X-gal/IPTG) in Escherichia coli.

Liquid Culture Selection: Maintains selective pressure in shaking cultures to prevent plasmid loss in Escherichia coli.

Recombinant Protein Expression Workflows: Supports maintenance of AmpR expression vectors in Escherichia coli during induction and growth.

Plasmid DNA Preparation: Ensures high-yield propagation of AmpR plasmids prior to mini/midi/maxiprep from Escherichia coli.

Library Construction and Screening: Provides selection for AmpR libraries (e.g., directed evolution, CRISPR cloning) in Escherichia coli.

General Antimicrobial in Microbiology Media: Inhibits growth of β-lactam–susceptible bacteria to reduce background contamination in bacterial workflows.