Antibiotic Selection in Bacterial Culture: Selects for bla (β-lactamase) plasmid-bearing E. coli using a 50 mg/mL stock diluted to typical 50–100 µg/mL final concentration.

Plasmid Maintenance and Cloning: Maintains ampicillin-resistant plasmids during cloning, subculturing, and miniprep/maxiprep workflows in E. coli.

Recombinant Protein Expression: Ensures retention of expression plasmids in E. coli during induction and culture growth.

LB/Agar Plate Preparation: Supplementation of LB agar/broth with ampicillin for selection of transformed E. coli colonies.

Blue-White Screening: Used with X-gal/IPTG on LB–ampicillin plates to identify recombinant clones in E. coli.

Starter Cultures and Glycerol Stocks: Selection during overnight cultures and long-term storage of ampicillin-resistant E. coli clones.

Contamination Control in Bacterial Workflows: Helps suppress susceptible bacterial contaminants in media and buffers when appropriate.