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2T3094

TCID50 DNA Extraction Buffer. 50mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

60mL

Item Count

1 Bottle(s)

Price per Item

$15.00

Fill Volume

50 mL

2T3094

TCID50 DNA Extraction Buffer. 50mL, Sterile.

Quantity

Minimum is set to 25 for this product*

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Description
Formulation
Common Applications
Storage

TCID50 DNA Extraction Buffer, 50 mL is a lysis solution for preparing DNA from cell culture samples associated with TCID50 workflows.

A buffered system incorporating a chelating agent and a blend of ionic and non-ionic detergents facilitates membrane disruption and nucleic acid release for crude DNA preparations.

  • 2.33mM Tris-HCl
  • 2.33mM EDTA
  • 0.233% SDS
  • 0.581% Sodium Deoxycholate
  • 1.030% Tween-20
  • DNA Extraction from Cultured Cells: Detergent-mediated lysis and EDTA chelation enable efficient genomic DNA release from mammalian and insect cells.

  • TCID50/qPCR Workflows for DNA Viruses: Rapid lysis of infected cell pellets or supernatants to release viral DNA for quantitative PCR-based titering.

  • Sample Preparation for PCR/qPCR/ddPCR: Produces lysates suitable for downstream amplification after appropriate dilution or cleanup.

  • NGS Library Preparation Input: Extraction of DNA from cell culture samples for use in sequencing workflows.

  • Bacterial Cell Lysis for Genomic DNA: Effective disruption of organisms such as Escherichia coli to obtain DNA for molecular assays.

  • Enveloped Virus Disruption: SDS, sodium deoxycholate, and Tween-20 facilitate envelope solubilization for downstream DNA analysis.

  • Clinical/Environmental Swab Processing: Lysis of swab-collected material to release DNA for diagnostic or surveillance testing.

  • Pathogen Inactivation for DNA Analysis: Detergent and chelator mix aids in inactivating microbes while preserving nucleic acids for testing.

Storage Temperature
Room Temperature
Shelf Life
1095 days
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